Benzo[k,l]xanthene Lignan-Loaded Solid Lipid Nanoparticles for Topical Application: A Preliminary Study

Area of Science:

• Dermatology
• Nanotechnology
• Biochemistry

Background:

• Skin barrier is vulnerable to physical and chemical agents, increasing reactive oxygen species (ROS).
• Benzo[k,l]xanthene lignans (BXL) are potent antioxidants ideal for mitigating ROS effects.
• Topical disorders can arise from elevated ROS levels impacting skin health.

Purpose of the Study:

• Formulate BXL-loaded solid lipid nanoparticles (SLN-BXL) to enhance skin bioavailability and interaction.
• Investigate the protective impact of SLN-BXL against intracellular ROS generation in HFF-1 cells.
• Compare the efficacy of SLN-BXL against drug-free conditions in mitigating ROS.

Main Methods:

• SLN-BXL formulated using the Phase Inversion Temperature (PIT)/ultrasonication method.
• Physicochemical characterizations included size, zeta potential (ZP), polydispersity index (PDI), and encapsulation efficiency (%EE).
• In vitro experiments assessed biomembrane interaction, release kinetics, cell safety, and ROS attenuation.

Main Results:

• SLN-BXL exhibited a mean size of ~200 nm, PDI of 0.2, and ZP of -28 mV, remaining stable for three months.
• Encapsulation efficiency (%EE) was approximately 70%, with significant biomembrane interaction and double-action release.
• In vitro studies confirmed SLN-BXL are cell-safe and effectively reduce IL-2-induced high ROS levels.

Conclusions:

• The developed SLN-BXL formulation demonstrates excellent physicochemical properties and stability.
• SLN-BXL show significant potential for deep skin interaction and sustained release of BXL.
• The formulation is effective in attenuating ROS and can be considered a preventive remedy for ROS-induced skin disorders.