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Exploring RNA modifications, editing, and splicing changes in hyperuricemia and gout.

Chung-Ming Huang1,2,3, Yu-Chia Chen1,4,5, I-Lu Lai1,4

  • 1Center for Precision Medicine, China Medical University Hospital, Taichung, Taiwan.

Frontiers in Medicine
|September 23, 2022
PubMed
Summary

This study reveals that RNA modifications, editing, and splicing are altered in gout. These findings offer new insights into gout mechanisms and potential diagnostic and therapeutic strategies for this common metabolic disorder.

Keywords:
RNA editingRNA modificationsRNA splicingepigenomic regulationsgout

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Area of Science:

  • Biochemistry
  • Genetics
  • Molecular Biology

Background:

  • Hyperuricemia and gout are prevalent metabolic disorders linked to lifestyle changes and associated with renal and cardiovascular diseases.
  • Existing research primarily focuses on gout-associated genes and the roles of immune cells, with limited investigation into epigenomics.

Purpose of the Study:

  • To investigate the role of RNA modifications, editing, and splicing in the pathogenesis of gout.
  • To explore the clinical significance of these RNA-related processes in gout patients.

Main Methods:

  • Analysis of RNA modification, editing, and splicing in cell lines (THP-1, HEK293) treated with uric acid (UA) or monosodium urate (MSU).
  • Transcriptome sequencing to assess genome-wide RNA splicing and editing changes.
  • Examination of RNA modifications, editing, and splicing in patient samples.

Main Results:

  • UA or MSU treatment affected enzymes involved in RNA modifications and editing.
  • RNA alternative splicing and splicing factors were altered by UA or MSU.
  • Significant changes in RNA splicing and editing were observed in treated cell lines and in patients with gout.

Conclusions:

  • RNA modifications, editing, and splicing play significant roles in the development of gout.
  • These findings provide a foundation for understanding gout mechanisms at the RNA level.
  • The study may facilitate the development of novel diagnostic and therapeutic approaches for gout.