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DNA Methylation Analysis of Cnr1 Gene Promoter
Claudio D'Addario1, Mariangela Pucci2
1Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2022
Abstract:
DNA methylation pattern could be considered a biomarker to be exploited for the study and management of several human diseases. In this chapter, detailed protocols are provided for two experimental approaches used for quantitative methylation analysis of bisulfite converted DNA: methylation-specific PCR (MSP) and pyrosequencing.

