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Updated: Aug 27, 2025
06:28
Sample Preparation for Mass Cytometry Analysis
Published on: April 29, 2017
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Histochemistry in Advanced Cytometry: From Fluorochromes to Mass Probes.
Giuliano Mazzini1,2, Marco Danova3
1Institute of Molecular Genetics - CNR (National Research Council), Pavia, Italy. mazzi@igm.cnr.it.
Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2022
Summary
Fluorescence techniques revolutionized cell analysis, evolving into microfluorometry and flow cytometry. New probes like quantum dots and mass cytometry offer advanced multiparametric analysis with reduced crosstalk and increased marker capacity.
Area of Science:
- Biochemistry and Biophysics
- Analytical Chemistry
- Cell Biology
Background:
- Fluorescence has been pivotal in quantitative analysis across sciences for decades.
- Classical cytochemistry dyes and microphotometry evolved into fluorochromes and microfluorometry, enhancing sensitivity and accuracy.
- Flow cytometry, developed in the 1960s, significantly advanced cell analysis, particularly in clinical diagnostics.
Purpose of the Study:
- To review the evolution of fluorescence-based techniques in cytometry.
- To highlight the challenges and advancements in multiparametric analysis.
- To introduce novel probes and analytical approaches in cytometry.
Main Methods:
- Evolutionary review of microfluorometry and flow cytometry techniques.
- Discussion of fluorochromes, quantum dots, and mass cytometry as analytical probes.
- Analysis of advancements in multiparametric cell analysis.
Main Results:
- Microfluorometry offers simplicity, sensitivity, and accuracy in cell analysis.
- Flow cytometry, combined with monoclonal antibodies, enables robust multiparametric analysis.
- Quantum dots overcome crosstalk issues in multiparametric analysis.
- Mass cytometry enables compensation-free analysis of numerous markers.
Conclusions:
- Technological advancements have continuously improved cytometry's analytical capabilities.
- Quantum dots and mass cytometry represent significant progress in high-parameter cell analysis.
- Future cytometry likely involves novel markers beyond fluorescence for comprehensive analysis.
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