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Related Concept Videos

Redox Titration: Iodimetry and Iodometry01:23

Redox Titration: Iodimetry and Iodometry

2.4K
Iodometry and iodimetry are analytical methods used to determine the concentration of oxidizing or reducing agents using iodine. In iodometric titrations, the oxidizing analyte solution is usually acidified and treated with an excess of iodide ions, which generates an equivalent amount of iodine in equilibrium with triiodide. The released iodine is subsequently titrated directly against a standardized reducing agent. As the dilute iodine color becomes pale yellow, a few drops of freshly...
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Simple Staining Technique01:24

Simple Staining Technique

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OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
459
Special Staining Techniques01:13

Special Staining Techniques

210
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
210
Fixation and Sectioning01:03

Fixation and Sectioning

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
4.7K
Differential Staining Technique01:26

Differential Staining Technique

326
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
326
Redox Titration: Overview01:21

Redox Titration: Overview

3.3K
Redox titration is a chemical analysis technique used to determine the concentration of an unknown substance by measuring the electron transfer in a redox (reduction-oxidation) reaction. The process involves gradually adding a titrant with a known concentration of an oxidizing or reducing agent, to the analyte, the solution with an unknown concentration, until reaching the endpoint, which indicates the completion of the reaction between the two substances. Ensuring the analyte is in a single...
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Related Experiment Video

Updated: Aug 27, 2025

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
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Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy

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Staining Starch with Iodine Solution.

Shengnan Zhao1,2, Yinhui Ren1,2, Cunxu Wei3,4

  • 1Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou, China.

Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2022
PubMed
Summary
This summary is machine-generated.

Staining plant tissues with iodine solution reveals starch granule morphology. This method visualizes starch in leaves, pollen, seeds, and isolated starch for detailed analysis.

Keywords:
Iodine solutionLeafPollen grainSeedStarch

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Last Updated: Aug 27, 2025

Combining Histochemical Staining and Image Analysis to Quantify Starch in the Ovary Primordia of Sweet Cherry during Winter Dormancy
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Area of Science:

  • Plant Biology
  • Biochemistry
  • Microscopy

Background:

  • Starch is a crucial storage carbohydrate in plants, with properties varying by source.
  • Understanding starch granule characteristics is vital for plant science and agriculture.

Purpose of the Study:

  • To present methods for visualizing starch granules in various plant tissues using iodine staining.
  • To demonstrate the utility of iodine staining for analyzing starch morphology.

Main Methods:

  • Iodine staining of starch in intact plant tissues (leaf, pollen, seeds).
  • Iodine staining of isolated starch granules.
  • Microscopic observation of stained starch samples.

Main Results:

  • Specific binding of iodine to starch allows for clear visualization of granule shape and size.
  • The method is effective across diverse plant materials, including storage tissues and reproductive cells.
  • Morphological details of starch granules are readily apparent.

Conclusions:

  • Iodine staining provides a simple yet effective technique for studying starch granule characteristics in plants.
  • This method aids in the comparative analysis of starch from different plant resources and tissues.