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Updated: Aug 27, 2025
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
Published on: November 17, 2023
Microcontact printing of gold nanoparticle at three-phase interface as flexible substrate for SERS detection of
Hongying Li1, Haina Zhang1, Wei Luo1
1Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education; College of Chemistry and Chemical Engineering, Southwest University, Chongqing, PR China.
Abstract:
The rigidity of traditional solid-state surface-enhanced Raman spectroscopy (SERS) substrate hampers their application in the curved structure for nonplanar surface test and in-situ detection. Traditionally, the flexible Raman substrates are often prepared by transferring printing of patterned nanoparticles on the flexible materials such as polymer, paper, etc. However，the replicate patterns are often produced by high-cost instruments. In this study, a low-cost and flexible SERS substrate is prepared by using a microcontact printing technology to transfer three-phase-assembled nanoparticles on a polydimethylsiloxane film, which can stabilize the assembled nanoparticles. Combining with the endonuclease Nt.BbvCI assisted amplification method, a SERS biosensor is constructed for microRNA 21 (miRNA 21) assay. This platform presents a wide dynamic range (100 fM ∼1 nM), achieving a fabulous sensitivity with limit of detection of 11.96 fM for miRNA 21. Furthermore, after being bent 90° for 50 times, the Raman intensity of the flexible substrate shows a negligible change. This versatile flexible substrate exhibits considerable potential for SERS analysis, which also opens a new avenue for preparing flexible devices.
