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Aptasensor for Impedimetric Detection of Lysozyme
Ece Eksin1, Huseyin Senturk1, Arzum Erdem2
1Faculty of Pharmacy, Analytical Chemistry Department, Ege University, Bornova, Izmir, Turkey.
Methods in Molecular Biology (Clifton, N.J.)
|September 26, 2022
Summary
This study presents a novel aptasensor for detecting lysozyme (LYS) using aptamer-functionalized pencil graphite electrodes (PGEs). The developed sensor demonstrates high selectivity and a low limit of detection for LYS.
Area of Science:
- Biosensors
- Electrochemistry
- Analytical Chemistry
Background:
- Protein detection is crucial in various fields, including diagnostics and food safety.
- Developing selective and sensitive detection methods for specific proteins like lysozyme (LYS) remains a challenge.
- Electrochemical methods offer a promising platform for label-free protein sensing.
Purpose of the Study:
- To develop a novel impedimetric aptasensor for the detection of lysozyme (LYS).
- To immobilize aptamers onto pencil graphite electrodes (PGEs) without chemical agents or nanomaterials.
- To evaluate the selectivity and sensitivity of the developed aptasensor for LYS detection.
Main Methods:
- Aptamer immobilization on electrochemically activated PGEs.
- Electrochemical characterization using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).
- Impedimetric detection of LYS interaction with immobilized aptamers.
Main Results:
- Successful immobilization of aptamers on PGEs via electrochemical activation.
- EIS analysis confirmed aptamer-target protein interaction.
- Achieved a limit of detection (LOD) of 1.44 μg/mL (100.65 nM) for LYS.
- Demonstrated high selectivity against bovine serum albumin and thrombin.
Conclusions:
- The aptamer-immobilized PGEs provide a sensitive and selective platform for impedimetric LYS detection.
- Electrochemical activation offers a reagent-free method for aptamer immobilization.
- This aptasensor shows potential for practical applications in protein analysis.
