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Ready-to-Use OECT Biosensor toward Rapid and Real-Time Protein Detection in Complex Biological Environments.

Shouyan Zhang1,2,3, Chunyang Xia1,2,3, Jun Wang1,2,3

  • 1School of Materials Science and Engineering, Shanghai University, 99 Shangda Road, Baoshan, Shanghai 200444, China.

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|April 28, 2025
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Summary
This summary is machine-generated.

This study presents an antifouling organic electrochemical transistor (OECT) sensor for rapid point-of-care testing. The novel zwitterionic OECT achieves ultrasensitive C-reactive protein detection with minimal interference, enabling real-time diagnostics.

Keywords:
C-reactive proteinantifoulingbiosensororganic electrochemical transistorpoly(3,4-ethylenedioxythiophene)polyphosphorylcholine

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Analytical Chemistry

Background:

  • Organic electrochemical transistors (OECTs) offer sensitive detection for point-of-care testing (POCT) in aqueous environments.
  • Biological fouling on OECTs from complex samples compromises accuracy by obscuring weak biomarker signals.
  • Nonspecific interactions lead to reduced sensitivity and potential false detections in biosensing.

Purpose of the Study:

  • To develop an intrinsically antifouling OECT sensor for robust biomarker detection in complex biological samples.
  • To enhance OECT performance and sensitivity through surface modification.
  • To achieve rapid, real-time detection of C-reactive protein (CRP) for POCT applications.

Main Methods:

  • Modification of OECT gate and channel interfaces with phosphorylcholine-functionalized poly(3,4-ethylenedioxythiophene) (PEDOT-PC).
  • Leveraging the inherent mixed electron-ion conductivity and zwitterionic nature of the modified material.
  • Assessing the sensor's performance for C-reactive protein (CRP) detection in the presence of interfering proteins.

Main Results:

  • The zwitterionic OECT demonstrated ultrasensitive CRP detection with a limit of detection of 0.11 pg/mL, mediated by calcium ions.
  • Enhanced transconductance and simultaneous sensing via gate and channel interfaces contributed to exceptional sensitivity.
  • The sensor achieved the fastest sample-to-result time (≤60 s) for protein detection, with no interference from common nonspecific proteins.

Conclusions:

  • The intrinsically antifouling zwitterionic OECT provides a robust platform for sensitive and rapid biomarker detection.
  • This technology offers a reliable and efficient solution for point-of-care diagnostics in complex biological environments.
  • The developed OECT sensor enables precise, real-time monitoring of biomarkers like CRP.