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Spectral Preprocessing Combined with Deep Transfer Learning to Evaluate Chlorophyll Content in Cotton Leaves
Qinlin Xiao1,2, Wentan Tang1,2, Chu Zhang3
1College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China.
Plant Phenomics (Washington, D.C.)
|September 26, 2022
Summary
Deep transfer learning with spectral preprocessing effectively estimates cotton chlorophyll content across varieties. This approach overcomes model variations, offering a cost-effective solution for field nutritional status evaluation.
Area of Science:
- Agricultural Science
- Spectroscopy
- Machine Learning
Background:
- Accurate chlorophyll content assessment is crucial for cotton's nutritional and physiological evaluation.
- Hyperspectral technology and multivariate analysis are common for chlorophyll detection, but models often lack transferability across different cotton varieties or batches.
- Variations in samples and measurement conditions necessitate robust and adaptable chlorophyll estimation methods.
Purpose of the Study:
- To explore the feasibility of using spectral preprocessing combined with deep transfer learning for effective model transfer in chlorophyll content estimation.
- To investigate the performance of a self-designed convolutional neural network (CNN) for chlorophyll detection and model transfer tasks.
- To identify optimal spectral preprocessing techniques for enhancing model transferability.
Main Methods:
- Seven spectral preprocessing methods were evaluated.
- A self-designed convolutional neural network (CNN) was developed for model building and transfer learning via fine-tuning.
- The combination of first-derivative (FD) and standard normal variate transformation (SNV) was identified as the best preprocessing approach.
Main Results:
- The fine-tuned CNN, using FD + SNV preprocessed spectra, significantly outperformed conventional Partial Least Squares (PLS) and Support Vector Machine Regression (SVR) models on the target dataset.
- Even with a smaller target dataset, the fine-tuned CNN demonstrated superior performance compared to conventional models, achieving satisfactory results.
- Ensemble preprocessing coupled with deep transfer learning proved effective for estimating chlorophyll content across different cotton varieties.
Conclusions:
- Ensemble spectral preprocessing combined with deep transfer learning offers a viable and effective strategy for transferring chlorophyll content estimation models between different cotton varieties.
- This approach provides a cost-effective alternative to developing new models for each batch or variety, enhancing the practicality of field-based cotton nutritional status evaluation.
- Deep transfer learning presents a promising avenue for robust and adaptable hyperspectral data analysis in agriculture.

