Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bioluminescent Probes for Multiplexed RNA Imaging.

Journal of the American Chemical Society·2026
Same author

Fluorescent Protein Chromophore-Based Luciferins for Bioluminescence Imaging.

Chemical & biomedical imaging·2025
Same author

Semisynthetic, Multicolor Probes for Bioluminescence Imaging.

ACS omega·2025
Same author

Continuous Hypermutation and Evolution of Luciferase Variants.

ACS chemical biology·2025
Same author

ESPRESSO: spatiotemporal omics based on organelle phenotyping.

Nature methods·2025
Same author

Mandibular swelling in a cat.

Journal of the American Veterinary Medical Association·2025
Same journal

Neutral Amphiphiles Boost Transfection Efficiency and Reduce Inflammation in Polymer Micelle-Mediated mRNA Delivery.

Bioconjugate chemistry·2026
Same journal

Surfactant-Mediated Buchwald-Hartwig Coupling of Aliphatic Amines for the Synthesis of DNA-Encoded Libraries.

Bioconjugate chemistry·2026
Same journal

Artificial Intelligence for Discovery in Life Sciences.

Bioconjugate chemistry·2026
Same journal

Iron Single Atom Nanozyme-Mediated GPX4 Inhibitor Delivery for Self-Enhanced Ferroptosis.

Bioconjugate chemistry·2026
Same journal

SpyCatcher-Engineered Ferritin Nanocages Enable Dual-Receptor Targeting for Enhanced Glioma Therapy.

Bioconjugate chemistry·2026
Same journal

One-Pot Synthesis of Functionalized Coumarin Fluorophores Enables Rapid Access to Live-Cell Bioorthogonal Labeling and Microenvironmental Sensing Agents.

Bioconjugate chemistry·2026
See all related articles

Related Experiment Video

Updated: Aug 27, 2025

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

22.5K

Generalized Bioluminescent Platform To Observe and Track Cellular Interactions.

Kevin K Ng1, Jennifer A Prescher1,2,3

  • 1Departments of Pharmaceutical Sciences, University of California, Irvine, California 92697, United States.

Bioconjugate Chemistry
|September 27, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed Luminescence to Observe and Track Intercellular Interactions (LOTIIS), a novel platform for visualizing cell-to-cell communication. LOTIIS uses split luciferase fragments to detect and track interacting cells in complex biological environments.

More Related Videos

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

4.7K
Bioluminescent Bacterial Imaging In Vivo
05:06

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

15.4K

Related Experiment Videos

Last Updated: Aug 27, 2025

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
10:38

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

22.5K
Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

4.7K
Bioluminescent Bacterial Imaging In Vivo
05:06

Bioluminescent Bacterial Imaging In Vivo

Published on: November 4, 2012

15.4K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Bioimaging

Background:

  • Cell-to-cell communication is vital for biological processes, including development and cancer.
  • Existing imaging methods struggle with thick tissues and complex biological environments.
  • New tools are needed to visualize intercellular interactions in situ.

Purpose of the Study:

  • To introduce a novel imaging platform, Luminescence to Observe and Track Intercellular Interactions (LOTIIS).
  • To enable visualization and tracking of cell-to-cell proximity and interactions.
  • To overcome limitations of current imaging strategies in complex biological settings.

Main Methods:

  • Utilized split fragments of a luciferase enzyme that reassemble upon cell proximity.
  • Designed one fragment to be secreted by 'sender' cells and the other by 'receiver' cells.
  • Incorporated a single chain variable fragment (scFv)-peptide interaction to facilitate reporter assembly and localized light production.

Main Results:

  • Demonstrated that LOTIIS can rapidly label cells in close proximity.
  • Showed that labeling is dependent on time and distance between cells.
  • Confirmed compatibility with bioluminescence resonance energy transfer (BRET) probes for multiplexed imaging.

Conclusions:

  • LOTIIS provides a robust platform for tracking cellular interactions.
  • The system enables visualization of cell proximity in a time- and distance-dependent manner.
  • This technology has broad applications for studying cell-cell communication in various biological contexts.