Related Experiment Video
Updated: Aug 27, 2025
TAPE: A Biodegradable Hemostatic Glue Inspired by a Ubiquitous Compound in Plants for Surgical Application
Published on: June 8, 2016
Contamination of Dentin with Hemostatic Agents - Is EDTA a Valuable Decontaminant before Using a Self-etch Universal
Purpose:
To investigate the effects of dentin decontamination procedures with ethylenediaminetetraacetic acid (EDTA) after contamination with two hemostatic agents, ViscoStat (VS) and ViscoStat Clear (VSC), on the microtensile bond strength (μTBS) of two different universal adhesives, before and after thermocycling (TC).
Materials And Methods:
Dentin surfaces of 100 human caries-free molars were either contaminated with one of the hemostatic agents or contaminated and then decontaminated with EDTA before the universal adhesives Scotchbond Universal Adhesive (SBU) or Prime & Bond Active (PBA) were applied in self-etch mode. Composite buildups were made and the teeth were sectioned into sticks (n = 90). Half of them immediately underwent the μTBS test, the other half after aging via TC. The data were statistically analyzed using Welch's ANOVA and the Games-Howell post-hoc test (p < 0.05).
Results:
Significant differences were observed between the groups (p < 0.001). When the dentin surface was contaminated with VSC, TC significantly reduced the mean bond strength, regardless of the universal adhesive. Decontamination with EDTA showed a significant decrease in bond strength after VS contamination and SBU application. The fracture analysis showed mainly adhesive fractures (78.8%) in all test groups.
Conclusion:
As EDTA application did not significantly increase the μTBS of either universal adhesive in self-etch mode in-vitro, it cannot be recommended as a decontaminant.
More Related Videos
07:21Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion
Published on: July 7, 2023
07:07Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
Related Concept Videos
01:19Masking and Demasking Agents
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
01:22EDTA: Chemistry and Properties
01:26EDTA: Auxiliary Complexing Reagents
01:18Effects of EDTA on End-Point Detection Methods
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
01:23EDTA: Indirect and Alkalimetric Titration
01:30EDTA: Direct, Back-, and Displacement Titration
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...