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Updated: Aug 27, 2025

Human Ex vivo Wound Model and Whole-Mount Staining Approach to Accurately Evaluate Skin Repair
Published on: February 17, 2021
[Thioredoxin in fibroblasts of human dermis in the process of aging.]
A G Gunin1, N N Golubtzova1, V U Emelianov1
1Chuvash State University, 15 Moscovski pr., Cheboksary 428015, Russian Federation,
Abstract:
The aim of this work was to examine the content of thioredoxin in fibroblasts of human dermis from the development until deep aging (from 20 weeks of pregnancy until 85 years old), and defining of a role of thioredoxin in age-dependent changes in the number of fibroblasts in the dermis. Thioredoxin, proliferating cells nuclear antigen (PCNA), marker of fibroblasts vimentin were detected with indirect immunohistochemical technique. Results showed that portion of fibroblasts with positive staining for thioredoxin in the dermis is increased from 20 weeks of pregnancy until 85 years old. Most expressed age related increase in portion of thioredoxin positive dermal fibroblasts (more than 9 times) is present form birth until 20 years as compared to antenatal period. General number and percent of PCNA positive fibroblasts in dermis are decreased with age with more expressed changes until 40 years old. Correlation analysis showed that age dependent decrease in the number of fibroblasts and their proliferative activity is significantly associated with increase in thioredoxin positive fibroblasts in dermis. Results allow to suggest that thioredoxin play a role in age dependent decrease in the number of fibroblasts and their proliferation in human dermis.
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