Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pre-Plexal Extension of Parsonage-Turner Syndrome With Nerve Root Involvement on Needle Electromyography.

Journal of clinical neuromuscular disease·2026
Same author

Recapitulating patient-to-patient colorectal cancer tumor heterogeneity using patient-derived xenograft cells in an engineered tissue model.

Acta biomaterialia·2026
Same author

Sotatercept Reverses SIN3a Deficiency-Driven PAH by Reprogramming BMPR2/TGF-β-HIF-1α Signaling Pathways.

bioRxiv : the preprint server for biology·2026
Same author

Non-invasive biomechanical characterization of embryos using microfluidic cantilevers.

European biophysics journal : EBJ·2026
Same author

Recapitulating Patient-to-Patient Colorectal Cancer Tumor Heterogeneity Using Patient-Derived Xenograft Cells in an Engineered Tissue Model.

bioRxiv : the preprint server for biology·2025
Same author

Systems analysis reveals neuregulin-1 control of cardiomyocyte size and shape mediated by distinct PI3K and p38 pathways.

bioRxiv : the preprint server for biology·2025
Same journal

Hydrogel-Encapsulated Primed MSCs Enhance Regeneration in Full-Thickness Porcine Burn Wounds.

Tissue engineering. Part A·2026
Same journal

Unidirectional Porous Carbonate Apatite Fabricated by Gelatin-Based Freeze Casting for Bone Regeneration.

Tissue engineering. Part A·2026
Same journal

Regenerative Nanoscaffolds for Chronic Tympanic Membrane Perforation: From Bench to Clinical Translation.

Tissue engineering. Part A·2026
Same journal

Impact of IFN-γ-Pretreated Umbilical Cord Mesenchymal Stem Cells Implanted in Mesh on Pelvic Organ Prolapse.

Tissue engineering. Part A·2026
Same journal

The Driving Force of Hierarchical Collagen Fiber Formation: A Review of Tendon, Ligament, and Meniscus Mechanobiology.

Tissue engineering. Part A·2026
Same journal

A Nondestructive Raman Spectral Method for Temporal Tracking of Articular Cartilage Maturation.

Tissue engineering. Part A·2026
See all related articles

Related Experiment Video

Updated: Aug 27, 2025

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.6K

Human Induced Pluripotent Stem Cell Encapsulation Geometry Impacts Three-Dimensional Developing Human Engineered

Morgan E Ellis1, Bryana N Harris1, Mohammadjafar Hashemi1

  • 1Department of Chemical Engineering, Auburn University, Auburn, Alabama, USA.

Tissue Engineering. Part A
|September 28, 2022
PubMed
Summary
This summary is machine-generated.

Altering the shape of engineered cardiac tissue during formation improves cardiomyocyte maturation. Rectangular shapes promote better tissue homogeneity and advanced cellular features, simplifying cardiac tissue engineering.

Keywords:
cardiac differentiationengineered heart tissuehuman induced pluripotent stem cellstissue geometry

More Related Videos

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

6.7K
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

601

Related Experiment Videos

Last Updated: Aug 27, 2025

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair
05:05

Preparation of Mesh-Shaped Engineered Cardiac Tissues Derived from Human iPS Cells for In Vivo Myocardial Repair

Published on: June 9, 2020

5.6K
Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
10:37

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells

Published on: March 14, 2021

6.7K
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

601

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Cardiovascular Research

Background:

  • Cardiac tissue engineering aims to address cardiovascular disease burdens.
  • Current methods for engineered cardiac tissue (3D-ECT) maturation are complex, often requiring cell selection or external pacing.
  • Direct differentiation from human induced pluripotent stem cells (hiPSCs) offers a simplified approach.

Purpose of the Study:

  • To investigate the impact of initial encapsulation geometry on 3D-ECT production and cardiomyocyte (CM) maturation.
  • To simplify the 3D-ECT formation process by directly differentiating hiPSCs.
  • To enhance CM maturation without exogenous stimulation.

Main Methods:

  • Encapsulating hiPSCs in poly(ethylene glycol)-fibrinogen within three geometries: disc-shaped microislands, squares, and rectangles.
  • Subjecting encapsulated cells to established cardiac differentiation protocols.
  • Analyzing CM populations, gene expression, tissue heterogeneity, myofibrillar alignment, Z-line formation, and contractile properties.

Main Results:

  • All geometries yielded similar CM populations (∼65%) and gene expression profiles.
  • Rectangular tissues exhibited reduced heterogeneity and enhanced features of CM maturation, including myofibrillar alignment and Z-line formation.
  • Rectangular 3D-ECTs demonstrated significantly higher anisotropic contractile properties compared to square and microisland tissues.

Conclusions:

  • Initial encapsulation geometry is a critical factor in improving 3D-ECT production and CM maturation.
  • Rectangular tissue geometry offers a straightforward method to enhance cardiac maturation, reducing the need for complex post-differentiation manipulations.
  • This approach has potential applications in bioprinting and cardiac drug testing.