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Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
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Zearalenone lactonase: characteristics, modification, and application
Yuanyuan Fang1, Zhenxia Zhang1, Wei Xu2
1State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
Applied Microbiology and Biotechnology
|September 29, 2022
Summary
Enzymatic detoxification using zearalenone lactonase (ZEN lactonase) offers a promising solution for eliminating this widespread fungal toxin. This review explores microbial ZEN lactonases for safe and effective food safety applications.
Area of Science:
- Biotechnology
- Food Science
- Environmental Science
Background:
- Zearalenone (ZEN) is a prevalent fungal toxin contaminating global food supplies, posing significant health risks.
- Conventional detoxification methods for ZEN are often inefficient and produce harmful byproducts.
Purpose of the Study:
- To provide a comprehensive review of microbial ZEN lactonases for ZEN detoxification.
- To discuss the gene mining, characterization, modification, and application of ZEN lactonases.
- To highlight the potential and challenges of ZEN lactonases in industrial settings.
Main Methods:
- Literature review focusing on microbial ZEN lactonases.
- Analysis of enzymatic properties, catalytic mechanisms, and reaction conditions.
- Examination of molecular modification strategies and biotechnological applications.
Main Results:
- ZEN lactonases exhibit high activity under mild conditions, producing non-toxic byproducts.
- Various microbial sources and engineered variants of ZEN lactonases have been identified.
- Enzymatic degradation offers a superior alternative to traditional ZEN detoxification methods.
Conclusions:
- Microbial ZEN lactonases are highly effective biocatalysts for degrading zearalenone.
- Further research into molecular modification can enhance ZEN lactonase efficiency for industrial use.
- Enzymatic bioremediation presents a sustainable solution for managing ZEN contamination in food and feed.
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