Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells
Atsushi Kunii1, Takashi Yamamoto1, Tetsushi Sakuma2
1Division of Integrated Sciences for Life, Graduate School of Integrated Sciences for Life, Hiroshima University, Hiroshima, Japan.
Abstract:
Genome editing technologies can be diverted into artificial transcription activators. In particular, researchers have improved dCas9-based technologies by tandem-fusing or trans-accumulating effector domains. Previously, we developed a hierarchical effector accumulation system named "TREE," enabling robust activation of target genes even when strongly silenced. In this chapter, we describe our protocol to design, construct, and validate the TREE-mediated target gene activation in cultured human cells.
More Related Videos
10:01An Efficient Strategy for Generating Tissue-specific Binary Transcription Systems in Drosophila by Genome Editing
Published on: September 19, 2018
06:10Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates
Published on: May 9, 2025
