Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.1K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.1K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

229
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
229

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Capillary bundling of microtubules by condensates.

bioRxiv : the preprint server for biology·2026
Same author

Power-dependent single-molecule dynamics of dark quencher blinking in QSY9/Cy3B: Diffusion-binding experiment and theory.

The Journal of chemical physics·2026
Same author

Topological defects promote layer formation in <i>Myxococcus xanthus</i> colonies.

Nature physics·2025
Same author

Capillary interactions drive the self-organization of bacterial colonies.

Nature physics·2025
Same author

A glyoxal-specific aldehyde signaling axis in Pseudomonas aeruginosa that influences quorum sensing and infection.

Nature communications·2025
Same author

Tracking Spatially Heterogeneous Dynamics of Single Nanoparticles Near Liquid-Solid Interfaces.

The journal of physical chemistry. B·2025

Related Experiment Video

Updated: Aug 27, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K

Multicolor multifocal 3D microscopy using in-situ optimization of a spatial light modulator.

M Junaid Amin1,2,3, Tian Zhao1, Haw Yang4

  • 1Department of Chemistry, Princeton University, Princeton, NJ, 08544, USA.

Scientific Reports
|September 29, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a multicolor multifocal microscopy technique using a Spatial Light Modulator (SLM) for uniform 3D imaging of multiple colors. This advance enables faster, more detailed visualization of complex biological samples and processes.

More Related Videos

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.4K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Related Experiment Videos

Last Updated: Aug 27, 2025

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K
A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

25.4K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.1K

Area of Science:

  • Biophotonics
  • Microscopy
  • Cell Biology

Background:

  • Multifocal microscopy achieves high-speed 3D imaging using multifocal gratings.
  • Standard gratings cause non-uniform illumination for multicolor imaging, limiting applications.
  • Existing methods struggle with simultaneous multicolor 3D visualization.

Purpose of the Study:

  • To develop a multicolor multifocal microscope with uniform subimage intensities across multiple emission bands.
  • To overcome limitations of single-wavelength gratings in multicolor imaging.
  • To enable high-speed 3D imaging of multicolored samples.

Main Methods:

  • Implemented a Spatial Light Modulator (SLM) as a dynamic multifocal grating.
  • Utilized real-time, in-situ optimization of the SLM for grating control.
  • Demonstrated multicolor 3D imaging across three emission bands.

Main Results:

  • Achieved near-uniform multifocal subimage intensities across multiple emission wavelengths.
  • Acquired multicolor 3D volumes at high speeds (e.g., [Formula: see text] volumes/sec).
  • Successfully imaged multicolored particles and *Escherichia coli* interacting with cancer cells.

Conclusions:

  • The SLM-based multifocal microscope enables efficient multicolor 3D imaging.
  • The method is adaptable to different hardware, wavelengths, and band numbers.
  • This technique is valuable for studying fast, multi-species dynamic processes in 3D.