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Updated: Aug 27, 2025
14:23
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Published on: April 16, 2012
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Differentiation of Peripheral Treg
José Almeida-Santos1,2, Marie-Louise Bergman3, Jocelyne Demengeot3
1Instituto Gulbenkian de Ciência, Oeiras, Portugal. j.almeida-santos@imperial.ac.uk.
Methods in Molecular Biology (Clifton, N.J.)
|September 30, 2022
Summary
This study details methods for differentiating peripheral regulatory T cells (pTreg) from CD4+ T cells. Protocols involve adoptive transfer into mice with transient Treg depletion or oral ovalbumin delivery for antigen-specific pTreg induction.
Area of Science:
- Immunology
- Cell Biology
- T cell differentiation
Background:
- Peripheral regulatory T cells (pTregs) are crucial for immune homeostasis.
- Existing methods for pTreg differentiation require refinement for specific applications.
Purpose of the Study:
- To provide detailed protocols for differentiating polyclonal and monoclonal peripheral regulatory T cells (pTregs).
- To enable researchers to generate pTregs for immunological studies.
Main Methods:
- Isolation of naïve CD4+ T cells via flow cytometry.
- Induction of polyclonal pTregs through adoptive transfer into Foxp3-diphtheria toxin receptor transgenic mice with transient Treg depletion.
- Induction of monoclonal pTregs via oral ovalbumin administration in RAG-deficient DO11.10 mice.
- Optional magnetic enrichment of CD4+ T cells for analysis.
Main Results:
- Successful differentiation of polyclonal pTregs using adoptive transfer and transient Treg depletion.
- Effective induction of monoclonal pTregs in antigen-specific mouse models.
- Demonstration of flow cytometry for cell isolation and magnetic purification for sample enrichment.
Conclusions:
- Established protocols facilitate the generation of both polyclonal and monoclonal peripheral regulatory T cells.
- These methods support further research into the function and application of pTregs in immunology.
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