Real-Time Monitoring of Circadian Rhythms in the Eye

The mammalian eye harbors a full circadian system that controls several physiologically relevant functions within this organ. During the last two decades a few laboratories have developed transgenic animal models in which circadian rhythms can be monitored in real time using luciferase activity. The most famous transgenic mouse to record bioluminescence rhythms from different tissues and organs is the PERIOD2::LUCIFERASE (PER2::LUC) mouse developed by the Takahashi laboratory in early 2000. Since then, several studies have used this mouse model to dissect the mammalian circadian system by monitoring the circadian rhythm in the brain, the eye, and in many other peripheral organs and tissues. This chapter describes the methodology to record and analyze bioluminescence rhythms from the retina, retinal pigment epithelium, and cornea of PER2::LUC mice.