Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultrastructural and ionic studies in global ischemic dog brain.

K Kumar, M Goosmann, G S Krause

    Acta Neuropathologica
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Association of hyperglycaemia with hospital mortality in nondiabetic COVID-19 patients: A cohort study.

    Diabetes & metabolism·2021
    Same author

    Transition-transversion encoding and genetic relationship metric in ReliefF feature selection improves pathway enrichment in GWAS.

    BioData mining·2018
    Same author

    Neoplasms in the Adenohypophysis of Dogs: A Clinical and Pathologic Study.

    Pathologia veterinaria·2018
    Same author

    Functional Chromophobe Adenomas of the Canine Adenohypophysis: An Ultrastructural Evaluation of a Neoplasm of Pituitary Corticotrophs.

    Pathologia veterinaria·2018
    Same author

    The Ultrastructure and Histopathology of an Acidophil Adenoma of the Canine Adenohypophysis.

    Pathologia veterinaria·2018
    Same author

    An interaction quantitative trait loci tool implicates epistatic functional variants in an apoptosis pathway in smallpox vaccine eQTL data.

    Genes and immunity·2016

    During brain reperfusion after ischemia, significant shifts in tissue ions like calcium and sodium occur. These ionic changes, particularly calcium accumulation, contribute to neuronal damage and necrosis.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Global ischemic brain insult leads to cellular dysfunction.
    • Reperfusion following ischemia can exacerbate neuronal injury.

    Purpose of the Study:

    • To investigate the time course of ionic shifts in brain tissue during reperfusion.
    • To correlate ionic changes with ultrastructural alterations and neuronal damage.

    Main Methods:

    • A dog model of global ischemic brain insult was used.
    • Parietal cortex tissue was analyzed for Ca, Na, K, Mg, and Fe at various reperfusion time points (10 min, 2, 4, 8 h).
    • Ultrastructural studies and subcellular calcium localization were performed on in situ-fixed brains.

    Main Results:

    Related Experiment Videos

    • After 8 hours of reperfusion, calcium and sodium levels doubled, while potassium levels halved compared to controls.
    • Ultrastructural analysis revealed neuronal damage, including chromatin clumping and membrane disruption.
    • Significant calcium accumulation was observed in astrocytes, synaptic vesicles, dendrites, and mitochondria.

    Conclusions:

    • Ionic shifts during reperfusion indicate a failure in maintaining transmembrane gradients.
    • Enhanced calcium entry into neurons during reperfusion is a key cytotoxic mechanism leading to neuronal necrosis.
    • Ultrastructural damage correlates with ionic dysregulation observed during reperfusion.