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Published on: February 1, 2018
"Turn-off" photoelectrochemical aptasensor based on g-C3N4/WC/WO3 composites for tobramycin detection
Lu Qiao1, Yuan Zhu1, Tianjing Zeng2
1College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, China.
Abstract:
Tobramycin (TOB), as a widely used antibiotic, poses severe unpredictable risks to ecology and health. Here, a novel photoelectrochemical (PEC) aptasensor based on a "turn-off" PEC mode was constructed for TOB detection. The working electrode could be modified by g-C3N4/WC/WO3 composites and TOB aptamer probes in turn. The TOB aptamer probes could anchor on the g-C3N4/WC/WO3 through π-π stacking interaction to avoid interference from other modifications. When TOB was captured by the aptamer probes anchored on the modified fluorine-doped tin oxide (FTO) electrode, a decreased photocurrent was obtained owing to steric hindrance and hinder electron transfer. Under optimal conditions, 0.005-5 ng mL-1 of TOB could be detected with a detection limit as low as 2 pg mL-1. Meanwhile, actual samples were investigated as well. The proposed sensor shows high specificity, satisfactory detectability, great reproducibility, and may provide a new thought for detecting other pollutants.
