Development of Ni(II) resistant S. cerevisiae and its application: Adsorption study and modeling

Area of Science:

• Environmental Microbiology
• Bioremediation
• Biotechnology

Background:

• Nickel(II) contamination poses environmental risks.
• Developing efficient biosorbents for heavy metal removal is crucial.
• Saccharomyces cerevisiae offers potential for bioremediation applications.

Purpose of the Study:

• To develop a Saccharomyces cerevisiae strain resistant to high concentrations of nickel(II).
• To evaluate the biosorption capacity and removal efficiency of the resistant strain for nickel(II) from aqueous solutions.
• To characterize the physical and kinetic parameters influencing nickel(II) biosorption.

Main Methods:

• Microbial screening for nickel(II) tolerance and minimum inhibitory concentration (MIC).
• Biosorption experiments under varying physical conditions (pH, temperature, inoculum parameters).
• Characterization using SEM-EDAX and FTIR; kinetic and isotherm modeling (Langmuir, pseudo-second-order).

Main Results:

• Developed a nickel(II)-resistant Saccharomyces cerevisiae AJ208 with a MIC of 5500 mg/L.
• Achieved a maximum Langmuir adsorption capacity of 170.06 mg/g for nickel(II).
• Demonstrated efficient nickel(II) removal from 3000 mg/L initial concentration, with endothermic and spontaneous reaction kinetics.

Conclusions:

• Saccharomyces cerevisiae AJ208 is a highly promising strain for nickel(II) bioremediation.
• The study provides a robust methodology for developing and evaluating microbial biosorbents.
• The findings support the use of this yeast strain as a reference for nickel(II) removal from industrial wastewater.