A method for the continuous measurement of respiration and vapor uptake in rats

A method for determining the uptake of inhaled vapors in rats is described. The method uses gas chromatographic data to enable calculations of vapor concentration differences upstream and downstream from a rat exposed to the vapor by the nose-only route. Respiratory data are recorded simultaneously. The data can be used to determine the rate of uptake, uptake as a fraction of vapor inhaled and the effects of vapor inhalation on respiration.