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Effect and mechanisms of kaempferol against endometriosis based on network pharmacology and in vitro experiments
Junde Zhao1,2,3, Juntao Wang4, Jinxing Liu3
1Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan, 250012, China.
Abstract:
Endometriosis is a common gynecological disease, and its underlying mechanisms remain elusive. Patients are at a higher risk of recurrence after surgery or drug withdrawal. In this study, to identify a potentially effective and safe therapy for endometriosis, we screened potential target genes of kaempferol on endometriosis using network pharmacology and further validation. Network pharmacology showed kaempferol may suppress migratory and invasive properties by modulating the phosphoinositide 3-kinase (PI3K) pathway and its downstream target matrix metalloproteinase (MMP)9. Furthermore, in vitro experiments showed that kaempferol repressed the migration and invasion of endometrial cells, and this effect may be involved in mediating the PI3K-related genes, phosphatase and tensin homolog (PTEN) and MMP9. Network pharmacology and in vitro experiments showed that kaempferol, repressed the implantation of endometrial cells and formation of ectopic lesions by inhibiting migration and invasion and regulating PTEN and MMP9, which may be associated with the PI3K pathway.
Insights
Kaempferol may offer a new therapy for endometriosis by inhibiting cancer cell migration and invasion. This natural compound targets key pathways, potentially reducing disease recurrence and lesion formation.
Area of Science:
- Gynecological disease research
- Pharmacology and molecular biology
Background:
- Endometriosis is a prevalent gynecological condition with unclear mechanisms.
- High recurrence rates after treatment necessitate novel therapeutic strategies.
Purpose of the Study:
- To identify kaempferol's therapeutic targets in endometriosis using network pharmacology.
- To validate kaempferol's efficacy and underlying mechanisms in vitro.
Main Methods:
- Network pharmacology screening of kaempferol's targets in endometriosis.
- In vitro validation of kaempferol's effects on endometrial cell migration and invasion.
- Analysis of key molecular pathways including PI3K, PTEN, and MMP9.
Main Results:
- Kaempferol identified as a potential suppressor of endometrial cell migration and invasion.
- Kaempferol modulates the phosphoinositide 3-kinase (PI3K) pathway, affecting PTEN and MMP9.
- In vitro studies confirmed kaempferol's inhibition of endometrial cell implantation and ectopic lesion formation.
Conclusions:
- Kaempferol demonstrates therapeutic potential for endometriosis by inhibiting cell migration and invasion.
- Regulation of the PI3K pathway, PTEN, and MMP9 are key mechanisms for kaempferol's action.
- Kaempferol represents a promising agent for managing endometriosis and preventing recurrence.
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