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Updated: Aug 7, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Improvements in extraction yield by solid phase lipid extraction from liquid infant formula and human milk, and the
Yomi Watanabe1, Motoko Hirokawa2, Arisa Furukawa3
1Research Division of Biomaterials and Commodity Chemicals, Osaka Research Institute of Industrial Science and Technology, Osaka, Japan.
Abstract:
The Röse-Gottlieb method is one of the most widely used methods for extracting lipids from milk samples. However, we found that lipid recovery from liquid infant formula and human breast milk was lower than expected. Better lipid recovery from these liquid matrices was obtained by solid phase extraction using silica gel; ~10% more could be recovered from liquid infant formula and ruminant milk, and 25% more from human breast milk. However, the method is not recommended for lipid extraction from dried whole milk powders.

