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Ionic Liquid-Based Strategy for Predicting Protein Aggregation Propensity and Thermodynamic Stability.

Talia A Shmool1, Laura K Martin2, Richard P Matthews1

  • 1Department of Chemical Engineering, Imperial College London, South Kensington Campus, London SW7 2AZ, U.K.

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Summary
This summary is machine-generated.

Ionic liquids, specifically choline chloride, reduce protein aggregation and enhance stability in drug formulations. This strategy predicts and improves the storage of protein therapeutics under stress conditions.

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Area of Science:

  • Biochemistry and Biophysics
  • Materials Science
  • Pharmaceutical Sciences

Background:

  • Protein therapeutics face aggregation challenges during development and storage due to environmental stresses.
  • Ionic liquids (ILs), particularly biocompatible choline chloride ([Cho]Cl)-based ILs, show promise in preventing stress-induced protein conformational changes.
  • Predicting and mitigating protein aggregation is crucial for successful drug development.

Purpose of the Study:

  • To develop an ionic liquid-based strategy for predicting protein aggregation propensity and thermodynamic stability.
  • To evaluate the impact of choline chloride concentration on the stability of immunoglobulin G4 (IgG4) under various stress conditions.
  • To assess the long-term storage stability of IgG4 in choline chloride formulations.

Main Methods:

  • Systematic evaluation of IgG4 structural, thermal, and thermodynamic stability using dynamic light scattering, zeta potential, and variable temperature circular dichroism.
  • Molecular dynamics simulations to analyze IgG4 aggregation propensity and choline chloride-IgG4 interactions.
  • Re-evaluation of protein stability after 365 days of storage at 4 °C.

Main Results:

  • Increasing choline chloride concentration significantly reduced IgG4 aggregation propensity in fresh and stored samples.
  • Choline chloride demonstrated a protective effect against stress-induced protein misfolding and aggregation.
  • The study successfully predicted thermodynamic properties and aggregation propensity over extended storage periods.

Conclusions:

  • An ionic liquid-based strategy can effectively predict and enhance protein aggregation propensity and thermodynamic stability.
  • Choline chloride formulations offer a promising approach to increase the stability and storage life of protein therapeutics.
  • This predictive IL-based strategy has the potential to overcome major challenges in protein drug development.