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Protein tyrosine phosphatase PTPL1 suppresses lung cancer through Src/ERK/YAP1 signaling

Jing Wang1, Shuanghui Li1, Xiujuan Zhang1

  • 1Department of Pulmonary and Critical Care Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Thoracic Cancer
|October 4, 2022
PubMed
Abstract

Insights

Protein tyrosine phosphatase-L1 (PTPL1) suppresses lung cancer growth. Downregulating PTPL1 promotes cancer cell proliferation by activating the Src/ERK/YAP1 pathway, highlighting PTPL1 as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lung adenocarcinoma is a major global health concern.
  • Understanding the molecular mechanisms driving lung cancer progression is critical for developing effective treatments.

Purpose of the Study:

  • To investigate the functional role of protein tyrosine phosphatase-L1 (PTPL1) in lung adenocarcinoma.
  • To elucidate the molecular pathways regulated by PTPL1 in lung cancer.

Main Methods:

  • Utilized short hairpin RNA (shRNA) to knockdown PTPL1 in lung cancer cell lines.
  • Assessed cell proliferation using EdU, CCK8, and colony formation assays.
  • Examined tumor growth in a xenograft mouse model and analyzed molecular signaling pathways (Src/ERK/YAP1).

Main Results:

  • PTPL1 expression was found to be downregulated in lung cancer cell lines.
  • Knockdown of PTPL1 significantly increased lung cancer cell proliferation and tumor growth.
  • PTPL1 downregulation activated the Src/ERK pathway, leading to YAP1 nuclear translocation and activation.

Conclusions:

  • PTPL1 acts as a tumor suppressor in lung adenocarcinoma.
  • PTPL1 counteracts the Src/ERK/YAP1 signaling pathway, inhibiting lung cancer cell proliferation and growth.
  • PTPL1 represents a potential therapeutic target for lung cancer treatment.
Keywords:
PTPL1non-small cell lung cancerproliferation

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