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Intracellular Delivery of Micron-Sized Magnetic Particles through a Virus Infection Pathway

Xiayi Feng1,2, Ding Gao1, Yipeng Jing3,4

  • 1State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, PR China.

ACS Applied Materials & Interfaces
|October 5, 2022
PubMed

Insights

Virus-like particles (VLPs) enable micron-sized magnetic particles (M-MPs) to enter cells via a pathway mimicking viral infection. This breakthrough expands M-MP applications in biomedical imaging beyond traditional uptake cells.

Area of Science:

  • Biomedical Imaging
  • Nanotechnology
  • Cell Biology

Background:

  • Micron-sized magnetic particles (M-MPs) offer advantages like low toxicity and strong signals for biomedical imaging.
  • Current limitations include restricted cellular uptake, primarily by macrophages and phagocytes, hindering broader applications.
  • Virus-like particles (VLPs) present a potential solution for active intracellular delivery of M-MPs.

Purpose of the Study:

  • To elucidate the intracellular pathway of M-MPs when delivered by VLPs.
  • To investigate the mechanism of VLP-mediated M-MP internalization and transport.
  • To provide evidence for active M-MP transport into non-phagocytic cells.

Main Methods:

  • Utilized fluorescence co-localization techniques to track M-MP movement within cells.
  • Analyzed the intracellular trafficking pathway of M-MPs mediated by VLPs.
  • Compared M-MP pathway with known viral infection routes.

Main Results:

  • VLP-mediated M-MP internalization followed a pathway characteristic of viral infection.
  • The pathway involved caveolae-dependent endocytosis.
  • Observed microtubule-dependent transport and accumulation in the endoplasmic reticulum.

Conclusions:

  • VLPs facilitate active intracellular delivery of M-MPs into various cell types.
  • The mechanism involves a virus-like endocytic pathway.
  • This finding significantly broadens the potential applications of M-MPs in biomedical imaging and beyond.
Keywords:
fluorescence co-localizationintracellular pathwaymagnetic resonance imagingmicron-sized magnetic particlesvirus-like particles

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