GSDM gene polymorphisms regulate the IgE level in asthmatic patients.

Area of Science:

• Genetics
• Immunology
• Respiratory Medicine

Background:

• Gasdermin A (GSDMA) and Gasdermin B (GSDMB) are implicated in asthma across various populations.
• Elevated total serum immunoglobulin E (IgE) levels are characteristic of allergic disorders like asthma and allergic rhinitis.
• Immunoglobulin E (IgE) triggers the release of inflammatory mediators from mast cells in asthmatic patients.

Purpose of the Study:

• To investigate the association between single nucleotide polymorphisms (SNPs) in GSDMA and GSDMB genes and total serum IgE levels in Jordanian asthmatic children and adults.
• To explore the role of specific GSDMA (rs7212938) and GSDMB (rs7216389) polymorphisms in asthma pathogenesis within the Jordanian population.

Main Methods:

• Genetic analysis of GSDMA (rs7212938) and GSDMB (rs7216389) single nucleotide polymorphisms (SNPs).
• Utilized the polymerase chain reaction (PCR)/restriction fragment length polymorphism (RFLP) method for SNP analysis.
• Applied co-dominant, dominant, and recessive genetic models to genotype data.

Main Results:

• A significant correlation was observed between the GSDMB genetic SNP (rs7216389) and total serum IgE levels.
• A single minor allele in the GSDMB gene significantly alters IgE levels, suggesting a role in asthma pathogenesis in Jordanian children.
• The GSDMB polymorphism may confer a protective effect against asthma risk in children, but its association with high asthma risk in adults was not conclusive.

Conclusions:

• The GSDMB genetic SNP (rs7216389) is significantly associated with IgE levels in Jordanian asthma patients.
• No significant correlation was found between GSDMA genetic polymorphisms and IgE levels in either child or adult asthmatic patients.
• The GSDMB rs7216389 polymorphism shows potential as a biomarker for asthma-related IgE levels in the Jordanian population.