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Updated: Aug 26, 2025

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
Published on: November 20, 2021
Crystal structure of Campylobacter jejuni lipoprotein Cj1090c
Seonghee Paek1, Fumihiro Kawai1, Nilamani Venisetty1
1Department of Biology and Biochemistry, University of Houston, Houston, Texas, United States.
Abstract:
In Gram-negative bacteria, lipopolysaccharide (LPS) is an essential component of the asymmetric outer membrane (OM). LptE is an OM lipoprotein that forms a complex with the β-barrel OM protein, LptD. Incorporation of LPS into the OM outer leaflet is essential for bacterial viability, and mediated by the LptD/E complex. The genome of Campylobacter jejuni, a major foodborne pathogen, contains over 20 putative lipoproteins including Cj1090c. Here, we report the crystal structure of Cj1090c at 2.4 Å resolution, revealing structural evidence for LptE in C. jejuni. The analysis of this crystal structure, along with the genomic context, allows us to propose the C. jejuni LPS transport system for the first time, and permits for discussion of the features of the LptD/E complex of C. jejuni.
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