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Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

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Biosensors based on functional nucleic acids and isothermal amplification techniques.

Xiuen Cao¹, Chuanpin Chen¹, Qubo Zhu¹

¹Xiangya School of Pharmaceutical Sciences in Central South University, Changsha, 410013, Hunan, China.

|October 6, 2022

Summary

Functional nucleic acids and isothermal amplification techniques enhance biosensor performance. This review explores their principles and applications for improved detection sensitivity and range in biosensing.

Keywords:

Biosensor Functional nucleic acid Isothermal amplification technique

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Area of Science:

Biomedical Engineering
Molecular Biology
Analytical Chemistry

Background:

Functional nucleic acids offer design flexibility as biosensing recognition elements.
Isothermal amplification techniques provide high amplification efficiency for biosensors.
Combining these technologies significantly advances biosensor applications.

Purpose of the Study:

To review functional nucleic acids and isothermal amplification techniques for biosensing.
To detail their classification, principles, and application characteristics.
To summarize their key roles in enhancing biosensor performance.

Main Methods:

Literature review of functional nucleic acids.
Literature review of isothermal amplification techniques.
Analysis of combined applications in biosensing.

Main Results:

Functional nucleic acids provide versatile recognition capabilities.
Isothermal amplification ensures rapid and efficient signal generation.
Synergistic application leads to enhanced sensitivity and broader detection ranges.

Conclusions:

The integration of functional nucleic acids and isothermal amplification is crucial for next-generation biosensors.
This review provides a foundation for developing novel biosensing strategies.
Further research can optimize these systems for diverse diagnostic applications.