An automatic whole blood analyzer for renal function analysis with a centrifugal microfluidic device.

Area of Science:

• Biomedical Engineering
• Clinical Chemistry
• Point-of-Care Diagnostics

Background:

• Chronic kidney disease (CKD) is a rapidly growing global non-communicable disease (NCD).
• Accurate and rapid detection of renal function indices is crucial for managing CKD.
• Existing detection systems may lack portability and ease of use.

Purpose of the Study:

• To design a clinical biochemical detection system for rapid renal function index assessment.
• To develop a portable and user-friendly device for point-of-care testing.
• To enhance the detection of uric acid, creatinine, and urea using microfluidic technology.

Main Methods:

• A centrifugal microfluidic chip-based system was developed.
• An optical signal acquisition structure with a photosensor was employed.
• Enzymatic reactions for uric acid, creatinine, and urea detection were utilized.
• Signal amplification and software algorithms processed optical data based on the Beer-Lambert law.

Main Results:

• The system demonstrated good stability and accuracy in detecting renal function indices.
• The developed system is compact (21.3 cm × 16.5 cm × 19 cm) and portable.
• The system enables rapid detection of key biomarkers for kidney function.

Conclusions:

• The centrifugal microfluidic chip system offers a promising approach for rapid and accurate renal function testing.
• The portable and easy-to-operate design supports its potential for point-of-care applications.
• This technology holds significant promise for integrated diagnostics in future healthcare settings.