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Exploring the Two Herb Combination Strategy to Treat Injured PC12 Cells
Published on: November 18, 2022
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Rat Pheochromocytoma PC12 Cells in Culture
Pin-Chun Chen1, Chih-Tien Wang2
1Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan.
Methods in Molecular Biology (Clifton, N.J.)
|October 7, 2022
Abstract:
PC12 cells serve as a secretory cell model, especially suitable for studying the molecular mechanisms underlying fusion pore kinetics in regulated exocytosis of dense-core vesicles (DCVs). In this chapter, we describe a series of PC12 cell culture procedures optimized for real-time functional assays such as single-vesicle amperometry. In addition, these conditions have been widely used for single-cell biochemical assays such as the proximity ligation assay with immunostaining.
