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Updated: Aug 26, 2025
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Published on: April 16, 2014
Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopy
Chung Yu Chan1, Sue Han2, Xin Wang2
1National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. keith.chan@nih.gov.
Abstract:
Recent advances in stimulated emission depletion (STED) microscopy offer an unparalleled avenue to study membrane dynamics of exo- and endocytosis, such as fusion pore opening, pore expansion, constriction, and closure, as well as the membrane transformation from flat-shaped to round-shaped vesicles in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.
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