Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sensitive Skin Improvement Through Bioinformatics-Identified Cosmetic Ingredients That Regulate Transcriptome-Derived Biomarkers.

Biomolecules·2026
Same author

Metabolic adaptations of immunosuppressive cells in cancer: mechanisms and therapeutic targets.

Experimental & molecular medicine·2026
Same author

Comprehensive Proteomics and Machine Learning Analysis to Distinguish Follicular Adenoma and Follicular Thyroid Carcinoma from Indeterminate Thyroid Nodules.

Endocrinology and metabolism (Seoul, Korea)·2025
Same author

Corrigendum to "Preparation, characterization and in vivo antidiabetic effects of polysaccharides from Pachyrrhizus erosus" [Int. J. Biol. Macromol 114 (2018) 97-105].

International journal of biological macromolecules·2024
Same author

The Effects of Climate Change on the Distribution Pattern of Species Richness of Endemic Wetland Plants in the Qinghai-Tibet Plateau.

Plants (Basel, Switzerland)·2024
Same author

Research progress on antitumor effects of sea buckthorn, a traditional Chinese medicine homologous to food and medicine.

Frontiers in nutrition·2024

Related Experiment Video

Updated: Aug 26, 2025

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
12:01

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

Published on: December 31, 2012

13.9K

Live-cell imaging reveals the cellular dynamics in seed development.

Ji Min Shin1, Ling Yuan1, Tomokazu Kawashima2

  • 1Department of Plant and Soil Sciences, University of Kentucky, KY, USA; Kentucky Tobacco Research and Development Center, University of Kentucky, KY, USA.

Plant Science : an International Journal of Experimental Plant Biology
|October 7, 2022
PubMed
Summary

Live-cell imaging reveals complex cellular dynamics during flowering plant seed development. This advanced microscopy approach tracks embryo, endosperm, and seed coat formation in real-time, offering new insights into plant reproduction.

Keywords:
Confocal microscopyEmbryoEndospermFertilizationSeedSemi-in vivo live-cell imaging

More Related Videos

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.3K
Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
06:28

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Published on: May 16, 2025

440

Related Experiment Videos

Last Updated: Aug 26, 2025

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination
12:01

Long-term, High-resolution Confocal Time Lapse Imaging of Arabidopsis Cotyledon Epidermis during Germination

Published on: December 31, 2012

13.9K
Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations
07:14

Live Cell Imaging to Assess the Dynamics of Metaphase Timing and Cell Fate Following Mitotic Spindle Perturbations

Published on: September 20, 2019

8.3K
Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds
06:28

Preparation of Intact Tissue for Microscopic Analysis of the Endosperm Cell Layer in Developing and Mature Arabidopsis Seeds

Published on: May 16, 2025

440

Area of Science:

  • Plant Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Seed development involves intricate cellular processes within the embryo, endosperm, and maternal seed coat.
  • Key events include nuclear migration, cell polarity establishment, and multinuclear endosperm formation.
  • Inter-tissue communication is crucial for synchronizing developmental timing in seeds.

Purpose of the Study:

  • To review recent advancements in live-cell imaging techniques for studying seed development.
  • To highlight the application of semi-in vivo imaging in Arabidopsis thaliana for long-term observation.
  • To discuss future directions in visualizing dynamic seed formation processes.

Main Methods:

  • Utilizing advanced microscopy techniques for live-cell imaging.
  • Employing a less-invasive semi-in vivo approach for observing intact seeds.
  • Performing time-lapse analyses to capture dynamic cellular events during seed development.

Main Results:

  • Live-cell imaging enables detailed observation of cellular dynamics during seed development.
  • Semi-in vivo imaging provides extended observation periods for Arabidopsis thaliana seed development.
  • These methods allow for deciphering complex coordination and synchronization between seed tissues.

Conclusions:

  • Live-cell imaging is a powerful tool for understanding the complexities of seed development.
  • The semi-in vivo approach offers significant advantages for studying intact seed dynamics.
  • Future research will continue to leverage advanced imaging to unravel plant reproductive processes.