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Comprehensive ceRNA network for MACF1 regulates osteoblast proliferation.

Shanfeng Jiang1, Chong Yin1,2,3,4, Kai Dang1,2,3

  • 1Lab for Bone Metabolism, Xi'an Key Laboratory of Special Medicine and Health Engineering, Key Lab for Space Biosciences and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 710072, Xi'an, Shaanxi, China.

BMC Genomics
|October 7, 2022
PubMed
Summary
This summary is machine-generated.

Microtubule actin crosslinking factor 1 (MACF1) loss activates a competing endogenous RNA (ceRNA) network, impacting osteoblast differentiation. This study reveals key regulatory ncRNAs and mRNAs involved in bone formation.

Keywords:
Competing endogenous RNA (ceRNA)LnRNAMiRNAMicrotubule actin crosslinking factor 1 (macf1)Osteoblast proliferationTranscriptomic analysis

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Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Microtubule actin crosslinking factor 1 (MACF1) influences osteoblast proliferation and differentiation via non-coding RNAs (ncRNAs).
  • The specific role of MACF1 in regulating osteoblast differentiation through the competing endogenous RNA (ceRNA) network is not well understood.

Purpose of the Study:

  • To investigate the global expression profile of ceRNAs in MACF1 knockdown MC3TC‑E1 pre-osteoblast cells.
  • To elucidate the lncRNA-miRNA-mRNA network regulated by MACF1 in osteoblast differentiation.

Main Methods:

  • Profiling of messenger RNA (mRNA), microRNA (miRNA), and long ncRNA (lncRNA) expression in MACF1 knockdown cells.
  • Construction of a lncRNA-miRNA-mRNA network using bioinformatics approaches.
  • Pathway enrichment analysis to identify key biological pathways involved.

Main Results:

  • Differential expression of 547 lncRNAs, 107 miRNAs, and 376 mRNAs was observed, with significant alterations on chromosome 2.
  • A ceRNA network was constructed, identifying mir-7063 and mir-7646 as potent ncRNA regulators and mef2c as a key target gene.
  • Enrichment analysis highlighted fluid shear stress and atherosclerosis, p53 signaling, and focal adhesion pathways in osteoblast proliferation, with specific lncRNA-miRNA-mRNA interactions identified.

Conclusions:

  • MACF1 deletion triggers a comprehensive ceRNA network that governs osteoblast proliferation.
  • The study provides insights into the global expression profile of ceRNAs crucial for MC3TC‑E1 osteoblast differentiation upon MACF1 loss.