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Updated: Aug 26, 2025

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
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Comparative genomics of Trueperella pyogenes available in the genome database reveals multidrug resistance genomic

Kumaragurubaran Karthik1, Subbaiyan Anbazhagan2, Murugesan Ananda Chitra1

  • 1Central University Laboratory, Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu, India.

Journal of Global Antimicrobial Resistance
|October 9, 2022
PubMed
Abstract

Insights

Multidrug resistance genomic islands in Trueperella pyogenes were identified. These islands, found in swine strains, contain mobile genetic elements that may facilitate antimicrobial resistance gene transfer.

Area of Science:

  • Genomics
  • Microbiology
  • Veterinary Medicine

Background:

  • Trueperella pyogenes is an opportunistic pathogen affecting various animal species.
  • Understanding antimicrobial resistance mechanisms is crucial for combating infections.

Purpose of the Study:

  • To identify multidrug resistance genomic islands in Trueperella pyogenes.
  • To analyze genomes from the NCBI database and a newly isolated swine strain (TN_CUL_2020).

Main Methods:

  • Whole-genome sequencing of strain TN_CUL_2020 using the Illumina platform.
  • Comparative genomic analysis of publicly available T. pyogenes genomes.
  • Identification of antimicrobial resistance genes using ABRicate and genomic islands using IslandViewer 4.

Main Results:

  • Genomic islands (GIs) containing multidrug resistance genes were identified in several T. pyogenes strains, notably SH01, SH02, and TP1.
  • These GIs frequently included class I integrons and/or IS6100 elements.
  • Composite transposons of IS6100 and phage elements were also found within GIs, alongside toxin-antitoxin systems.

Conclusions:

  • Swine strains SH01 and SH02 exhibited multidrug resistance genes associated with class I integrons.
  • The presence of mobile genetic elements like integrons, insertional elements, type IV secretion systems, toxin-antitoxin systems, and phage elements within GIs likely promotes horizontal gene transfer of antimicrobial resistance.
Keywords:
Antimicrobial resistance genesComparative genomeGenomic islandsHorizontal transferIndiaTrueperella pyogenes

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