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Acoustic tethering of microorganisms.

M Rode1, A Bioue2, F Miano1

  • 1Centre for Ocean Life, National Institute of Aquatic Resources, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.

The Journal of Experimental Biology
|October 11, 2022
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Summary

Researchers developed a simple acoustic tethering setup for observing individual microorganisms under a light microscope. This method allows high-magnification behavioral studies without impacting cell motility, applicable to various microorganisms.

Keywords:
DinoflagellatesUltrasoundVideo-Microscopy

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Area of Science:

  • Microbiology
  • Biophysics
  • Microfluidics

Background:

  • Observing individual microorganisms' behavior is crucial for understanding cellular functions.
  • Traditional methods often face challenges in maintaining cell position and focus for high-magnification studies.
  • Acoustic manipulation offers a non-invasive approach for controlling microscopic particles and cells.

Purpose of the Study:

  • To present a novel, accessible setup for acoustically tethering microorganisms.
  • To demonstrate the application of this setup for detailed behavioral observations.
  • To assess the impact of acoustic tethering on microorganism motility and flagellar function.

Main Methods:

  • Construction of a simple acoustic tethering device using standard laboratory equipment.
  • Integration of the device with a conventional light microscope for high-magnification imaging.
  • Utilizing the setup to immobilize and observe the dinoflagellate Alexandrium minutum.

Main Results:

  • Successfully tethered individual microorganisms in the focal plane of the microscope.
  • Enabled high-magnification behavioral observations without altering flagellar beat frequencies.
  • Demonstrated the capability of the setup with Alexandrium minutum, a freely swimming dinoflagellate.

Conclusions:

  • The acoustic tethering setup provides a viable, non-invasive method for studying microorganism behavior.
  • The technique is adaptable for diverse cell types, including flagellates and ciliates of various sizes.
  • This method opens new avenues for research into microbial swimming kinematics and appendage motion.