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Updated: Aug 26, 2025
10:34
Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture
Published on: July 22, 2016
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Non-coding RNA network associated with obesity and rheumatoid arthritis
Eduardo Delabio Auer1, Denisson de Carvalho Santos2, Ismael Júnior Valério de Lima2
1Postgraduate Program in Genetics, Department of Genetics, Federal University of Paraná (UFPR), Centro Politécnico, Jardim das Américas, 81531-990 Curitiba, PR, Brazil.
Immunobiology
|October 11, 2022
Summary
Obesity and rheumatoid arthritis (RA) share altered non-coding RNAs (ncRNAs), including microRNAs (miRNAs) and long ncRNAs (lncRNAs). These ncRNAs correlate with disease activity and may offer new therapeutic targets for obese RA patients.
Area of Science:
- Epigenetics and Molecular Biology
- Immunology and Rheumatology
Background:
- Obesity induces epigenetic changes via non-coding RNAs (ncRNAs), heightening susceptibility to autoimmune and inflammatory diseases.
- Obese individuals with rheumatoid arthritis (RA) experience poorer clinical outcomes and treatment responses.
Purpose of the Study:
- To identify specific microRNAs (miRNAs) and long ncRNAs (lncRNAs) influencing RA in obese individuals.
- To understand the regulatory network of these ncRNAs in obese RA patients.
Main Methods:
- Systematic literature review of PubMed, Web of Science, and Scopus (last decade).
- Analysis of dysregulated miRNAs and lncRNAs in obesity and RA.
- Investigation of single nucleotide variants (SNVs) in lncRNA regions and their impact on miRNA binding and gene expression.
Main Results:
- Shared dysregulated ncRNAs in obesity and RA include miR-21, miR-143, miR-146a, miR-155, H19, and HOTAIR.
- Most identified ncRNAs correlated positively with clinical measures and RA disease activity.
- GWAS-identified SNVs affected miRNA binding sites and gene expression in adipocytes, particularly involving LINC01184 and GATA3-AS1.
Conclusions:
- A unique regulatory network of ncRNAs exists in obese RA patients.
- These ncRNAs represent potential novel therapeutic targets for managing co-occurring obesity and RA.
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