Related Experiment Video
Updated: Aug 26, 2025

Yeast Luminometric and Xenopus Oocyte Electrophysiological Examinations of the Molecular Mechanosensitivity of TRPV4
Published on: December 31, 2013
Species-Specific Activation of Transient Receptor Potential Ankyrin 1 by Phthalic Acid Monoesters
Yoko Mori1, Akira Aoki1, Yoshinori Okamoto1
1Faculty of Pharmacy, Meijo University.
Abstract:
Phthalic acid (PA) diesters are widely used in consumer products, as plasticizers, and are ubiquitous environmental pollutants. There is a growing concern about their adjuvant effect on allergic diseases. Although its precise mechanism remains unknown, possible involvement of transient receptor potential ankyrin 1 (TRPA1) has been suggested. Hence, in this study, the activation of human and mouse TRPA1s by a series of PA di- and monoesters was investigated using a heterologous expression system in vitro. Consequently, it was found that monoesters activated human TRPA1, where EC50 values were in the order of mono-hexyl > mono-heptyl > mono-n-octyl > mono-2-ethylhexyl > mono-isononyl and mono-isodecyl esters. Significant species differences in TRPA1 activation by PA monoesters were also discovered; PA monoesters activated human TRPA1 but not mouse TRPA1 in a concentration-dependent manner up to 50 µM. These findings suggest that PA esters may exert TRPA1-dependent adverse effects on humans, which have never been demonstrated in experimental animals.
Related Concept Videos
IP3/DAG Signaling Pathway
Activation and Inactivation of G Proteins
GPCRs Regulate Adenylyl Cylase Activity
G-Protein Gated Ion Channels
Sensory...
Mechanically-gated Ion Channels
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:

