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Updated: Aug 26, 2025
Preparing Irradiated and Marked Male Aedes aegypti Mosquitoes for Release in an Operational Sterile Insect Technique Program
Published on: March 12, 2021
Batch Rearing Aedes aegypti
Margot P Wohl1, Conor J McMeniman2,3
1W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland 21205, USA.
Abstract:
Standardized rearing methods for the yellow fever mosquito Aedes aegypti are critical to facilitate controlled laboratory studies. This protocol describes a batch rearing protocol for Aedes aegypti stocks that yields healthy eggs, larvae, pupae, and adults in the laboratory for long-term colony maintenance and experimental manipulation. Foundational principles for the rearing and containment of these life cycle stages, as well as steps for mating and blood feeding Aedes aegypti to yield viable eggs for continuous culture or storage, are detailed.
