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Related Experiment Video

Updated: Aug 25, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

Real-time dynamic single-molecule protein sequencing on an integrated semiconductor device.

Brian D Reed¹, Michael J Meyer¹, Valentin Abramzon

¹Quantum-Si, Inc., Guilford, CT 06437, USA.

Science (New York, N.Y.)

|October 13, 2022

Summary

This summary is machine-generated.

This study introduces a novel single-molecule protein sequencing method. It uses real-time amino acid recognition and cleavage for sensitive proteomic analysis and detection of modifications.

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Last Updated: Aug 25, 2025

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Published on: May 23, 2018

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Published on: August 26, 2009

Area of Science:

Biochemistry
Proteomics
Molecular Biology

Background:

Proteomic studies require sensitive methods for direct protein sequencing and quantification.
Detecting posttranslational modifications at the single-molecule level remains a challenge.

Purpose of the Study:

To develop a novel single-molecule protein sequencing technique.
To enable real-time digital quantification and modification detection of proteins.

Main Methods:

A dynamic approach using dye-labeled amino acid recognizers and aminopeptidases.
Real-time probing of single peptides on an integrated semiconductor chip.
Analysis of fluorescence intensity, lifetime, and binding kinetics for amino acid annotation.

Main Results:

Demonstrated successful single-molecule protein sequencing.
Identified amino acids and peptide sequences based on kinetic principles.
Showcased discrimination of single amino acid substitutions and posttranslational modifications.

Conclusions:

The developed method offers a sensitive and scalable platform for single-molecule proteomic studies.
This approach has the potential for accessible proteomic analysis.
Further development could significantly advance the field of proteomics.