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Updated: Aug 25, 2025

A Whole Mount In Situ Hybridization Method for the Gastropod Mollusc Lymnaea stagnalis
Published on: March 15, 2016
Maxiclavella and Praeclavella (Siphonostomatoida: Lernaeopodidae) new genera confirmed by molecular and morphological
Raul Castro-Romero1, Martin Miguel Montes2, Sergio Martorelli2
1Universidad de Antofagasta, Facultad de Ciencias del Mar, Depto. de Ciencias Acuáticas y Ambientales, Casilla 170, 1240000 Antofagasta, Chile.
Abstract:
The new genus Maxiclavella is proposed to accommodate Clavella simplex Castro Romero and Baeza Kuroki, 1985, which differs from Clavella species, including long and narrow cephalothorax, small bulla, and simple armature of the antenna and antennule. Praeclavella nasalis, new genus and species, was found parasitizing the olfactory sacs of Isacia conceptionis. Praeclavella nasalis could be differentiated from other Clavella species by a biramous antenna with an exopod shorter than the endopod, the base of the cephalothorax with a large lobular and suborbicular projection on each side, a suborbicular bulla, mandibles without secondary teeth, and a suboval male body type. Males of both genera fit well in the Clavella-clade male type, although they differ from each other in many aspects, mainly in the position of the buccal area, which is oriented ventrally in Maxiclavella and distally in Praeclavella nasalis, and in a genital process, which is present in the latter and absent in the former. Genetic distances of mtDNA COI and 28S rDNA supported the validity of the two new genera parasitizing the same host, I. conceptionis. Also Clavella-branch (Clavellinae Wilson), a key based genera on female specimens is presented.
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