MnO₂ nanosheets-triggered oxVB₁ fluorescence immunoassay for detection zearalenone

In this study, an alkaline phosphatase (ALP)-mediated fluorescence immunoassay for detecting zearalenone (ZEN) was established based on the oxVB₁ fluorescence signal modulated by MnO₂ nanosheets (MnO₂ NS). As the ALP-antibody content increased, more 2-phosphoascorbic acid (AAP) was hydrolyzed to ascorbic acid (AA) which destroyed the MnO₂ NS rapidly. In the lack of MnO₂ NS, VB₁ cannot be oxidized to oxVB₁ for emitting fluorescence. On the contrary, the fluorescence of oxVB₁ recovered slowly with the decrease of the ALP-antibody concentration. In the optimization condition, the detection limit of this method was 15.5 pg mL^-1. Moreover, the recovery of ZEN in real samples ranged from 94.24 % to 108.26 %, which indicated the remarkable accuracy and reliability of this approach. Meanwhile, the proposal of this fluorescence immunoassay provided a new possibility for detecting other targets by replacing antibodies and antigens.