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LncRNA MAFG-AS1-induced acute myeloid leukemia development via modulating miR-147b/HOXA9

Qiying Yao1, Li Zhang2, Zhengjuan Liu2

  • 1College of Basic Medical Sciences, Dalian Medical University, Dalian, 116027, Liaoning, China.

Environmental Science and Pollution Research International
|October 13, 2022
PubMed

Insights

Long non-coding RNA MAFG-AS1 promotes acute myeloid leukemia (AML) progression by sponging miR-147b and upregulating HOXA9. MAFG-AS1 acts as an oncogene in AML development.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in cancer development.
  • The specific role of MAFG-AS1 in acute myeloid leukemia (AML) remains unclear.

Purpose of the Study:

  • To investigate the function and mechanism of MAFG-AS1 in AML pathogenesis.
  • To determine the relationship between MAFG-AS1, miR-147b, and HOXA9 in AML.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess gene expression.
  • Cell counting kit-8 (CCK-8) assay and flow cytometry for cell proliferation and cycle analysis.
  • Luciferase reporter assays to elucidate molecular interactions.

Main Results:

  • MAFG-AS1 was significantly overexpressed in AML cells and patient samples.
  • MAFG-AS1 promoted AML cell proliferation, cell cycle progression, and epithelial-mesenchymal transition (EMT).
  • MAFG-AS1 sponged miR-147b, which was downregulated in AML, and HOXA9 was identified as a target of miR-147b.

Conclusions:

  • MAFG-AS1 functions as an oncogene in AML.
  • MAFG-AS1 accelerates AML progression by modulating the miR-147b/HOXA9 axis.
Keywords:
Acute myeloid leukemiaHOXA9MAFG-AS1miR-147b

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