Jellyfish Polysaccharides for Wound Healing Applications

Area of Science:

• Biomaterials Science
• Marine Biotechnology
• Dermatology

Background:

• Jellyfish are increasingly recognized for their potential in food, pharmaceutical, and biomedical applications.
• Marine collagen is one valuable product derived from jellyfish.
• This study focuses on jellyfish skin polysaccharides (JSP) with glycosaminoglycan (GAG) features.

Purpose of the Study:

• To extract and characterize jellyfish skin polysaccharides (JSP) from Rhizostoma pulmo.
• To evaluate the wound healing and antioxidant properties of these GAG-like polysaccharides.
• To explore their potential as functional components for biomedical skin treatments and pharmaceutical excipients.

Main Methods:

• Extraction and isolation of two main JSP fractions (RP-JSP1 and RP-JSP2) from Rhizostoma pulmo.
• Characterization of molecular weight and sugar composition using LC-MS analysis.
• In vitro evaluation of wound healing (scratch assay) and oxidative stress protection on fibroblast and keratinocyte cell lines.

Main Results:

• Two fractions of R. pulmo JSP (RP-JSPs) were isolated with average molecular weights of 121 kDa and 590 kDa.
• LC-MS confirmed the presence of typical GAG saccharides, including glucose, galactose, glucosamine, and galactosamine.
• Both RP-JSPs significantly promoted wound healing (80% scratch repair in two days) and protected cells from oxidative stress.

Conclusions:

• Rhizostoma pulmo jellyfish skin polysaccharides exhibit significant wound healing and antioxidant properties.
• These GAG-like polysaccharides are effective in promoting cell migration and proliferation.
• RP-JSPs show considerable potential for use in biomedical skin treatments and as pharmaceutical excipients.