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Updated: Aug 25, 2025

Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
Published on: September 13, 2022
Super-Resolution Imaging Reveals Dynamic Reticular Cytoophidia
Yi-Fan Fang1, Yi-Lan Li1, Xiao-Ming Li1
1School of Life Science and Technology, Shanghai Tech University, Shanghai 201210, China.
Abstract:
CTP synthase (CTPS) can form filamentous structures termed cytoophidia in cells in all three domains of life. In order to study the mesoscale structure of cytoophidia, we perform fluorescence recovery after photobleaching (FRAP) and stimulated emission depletion (STED) microscopy in human cells. By using an EGFP dimeric tag as a tool to explore the physical properties of cytoophidia, we find that cytoophidia are dynamic and reticular. The reticular structure of CTPS cytoophidia may provide space for other components, such as IMPDH. In addition, we observe CTPS granules with tentacles.
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