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Screening of Humic Substances Extracted from Leonardite for Free Radical Scavenging Activity Using DPPH Method.

Attila Csicsor1,2, Etelka Tombácz3

  • 1Doctoral School of Environmental Sciences, University of Szeged, Rerrich Béla tér 1., H-6720 Szeged, Hungary.

Molecules (Basel, Switzerland)
|October 14, 2022
PubMed
Summary
This summary is machine-generated.

Humic substances (HSs) possess antioxidant properties, but extraction methods significantly alter their effectiveness. This study reveals that different fractionation processes impact HS physico-chemical traits and free radical scavenging activity.

Keywords:
DPPHantioxidantextractionfulvic acidhimatomelanic acidhumic acidhumic substancesleonarditeradical scavenging activity

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Area of Science:

  • Environmental Chemistry
  • Organic Geochemistry
  • Biochemistry

Background:

  • Humic substances (HSs) are extensively studied organic compounds with known biological effects.
  • Emerging research highlights the antioxidant properties of HSs.
  • Previous studies often overlook the influence of extraction methods on HS antioxidant activity and spectral interference.

Purpose of the Study:

  • To investigate the impact of different extraction and fractionation methods on the antioxidant properties of HSs.
  • To characterize HS fractions and compare their antioxidant activity using the DPPH method.
  • To validate self-extracted HS samples against International Humic Substances Society (IHSS) standards.

Main Methods:

  • Extraction of HS fractions from leonardite using industrially scalable processes.
  • Characterization of HS fractions via elemental analysis, UV-Vis, and FT-IR spectroscopy.
  • Assessment of antioxidant activity using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay.

Main Results:

  • Fractionation methods influenced the elemental composition and spectral characteristics of HSs.
  • The study confirmed that self-extracted HS samples are comparable to IHSS standards.
  • Antioxidant activity varied among humic, fulvic, and himatomelanic acid fractions, demonstrating the effect of the extraction method.

Conclusions:

  • The extraction and fractionation processes significantly affect the physico-chemical properties of humic substances.
  • Extraction method is a critical factor influencing the free radical scavenging activity of HS fractions.
  • Understanding these variations is crucial for accurately assessing HS antioxidant potential.