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Updated: Aug 25, 2025

Fluorescence detection methods for microfluidic droplet platforms

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Low-cost programable stroboscopic illumination with sub-microsecond pulses for high-throughput microfluidic

Marko Tuljak¹, David Lajevec¹, Rok Štanc¹

¹Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia.

|October 14, 2022

Summary

This summary is machine-generated.

Researchers developed a low-cost, high-speed stroboscopic LED illumination system for microscopy. This system enables microsecond-level imaging for applications like microfluidics and cell deformability cytometry.

Keywords:

Arduino Deformability cytometry Microfluidics Stroboscope Teensy Velocity measurement

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Last Updated: Aug 25, 2025

Fluorescence detection methods for microfluidic droplet platforms

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Published on: December 10, 2011

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Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Area of Science:

Biophysics
Optical Engineering
Microfluidics

Background:

High-speed imaging is crucial for microfluidic applications.
Commercial systems for microsecond-level imaging are often prohibitively expensive for research labs.

Purpose of the Study:

To develop an affordable stroboscopic illumination system for transmitted-light microscopy.
To achieve image acquisition times on the order of 500 nanoseconds.

Main Methods:

A high-power LED was used as the light source for stroboscopic illumination.
The system was designed for easy mounting on standard inverted microscopes.
An Arduino-compatible microcontroller (Teensy 4.0) controlled illumination, with a Python GUI for parameter setting.

Main Results:

The developed system achieved exposure times of approximately 500 nanoseconds.
The system is compatible with standard industrial cameras.
It was successfully applied to cell phenotyping via deformability cytometry and droplet microfluidics.

Conclusions:

The low-cost stroboscopic LED illumination system provides a viable alternative for high-speed microscopy.
This system democratizes high-throughput imaging in microfluidic research and cell analysis.