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Related Concept Videos

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Related Experiment Video

Updated: Aug 25, 2025

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases
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Microarray data reveal potential genes that regulate triple-negative breast cancer.

Chi Pan1, Aihua Cong2, Qingtao Ni2

  • 1Department of General Surgery, Jiangsu Taizhou People's Hospital, Taizhou, China.

The Journal of International Medical Research
|October 14, 2022
PubMed
Summary

Triple-negative breast cancer (TNBC) research identified 18 upregulated genes, including CDK1. MiR-143-3p, a regulator of CDK1, was downregulated, suggesting both could be therapeutic targets for TNBC.

Keywords:
BioinformaticsCDK1cell cyclekey genesmiR-143-3ptriple-negative breast cancer

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Area of Science:

  • Oncology
  • Genomics
  • Molecular Biology

Background:

  • Triple-negative breast cancer (TNBC) presents significant challenges due to limited targeted therapies and poor prognoses.
  • The precise pathological mechanisms driving TNBC development are not fully understood.

Purpose of the Study:

  • To identify key genes and associated molecular pathways crucial for TNBC development.
  • To uncover potential therapeutic targets for TNBC.

Main Methods:

  • Screened Gene Expression Omnibus (GEO) database for transcriptome data to identify differentially expressed genes in TNBC.
  • Utilized Gene Ontology analysis to pinpoint involved genes and pathways.
  • Correlated gene and microRNA (miRNA) expression using multiple databases (miRDB, TargetScan, miRWalk, DIANA).
  • Validated expression of Cyclin-Dependent Kinase 1 (CDK1) and miR-143-3p in clinical TNBC samples.

Main Results:

  • Identified 18 significantly upregulated genes in TNBC, enriched in cell metabolism, division, mitochondria, and respiratory chain processes.
  • Established miR-143-3p as an upstream regulator of CDK1.
  • Clinical validation confirmed CDK1 upregulation and miR-143-3p downregulation in TNBC specimens.

Conclusions:

  • The study identified 18 upregulated genes associated with TNBC.
  • CDK1 and its regulatory miRNA, miR-143-3p, emerged as potential therapeutic targets for TNBC treatment.