Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

479
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
479
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.9K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.9K
IR Spectrometers01:25

IR Spectrometers

1.3K
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Role of Nitric Oxide, Ethylene, Hydrogen Sulfide and Their Interplay With Histone Acetylation in Plants.

Physiologia plantarum·2026
Same author

Lessons fromα-RuCl<sub>3</sub>for pursuing quantum spin liquid physics in atomically thin materials.

Journal of physics. Condensed matter : an Institute of Physics journal·2026
Same author

Terahertz spectral imaging for early assessment of frostbite injuries using the double Debye model and supervised machine learning.

Biomedical optics express·2026
Same author

Relationship between DNA methylation and ethylene in plants: A review.

Plant science : an international journal of experimental plant biology·2026
Same author

Polarization-Engineered Near-Field Generation Using a Hybrid Tip-Antenna System.

Small methods·2026
Same author

Genome-Wide Analysis of Tomato <i>SlCCD</i> Genes and the Role of <i>SlCCD11</i> in Enhancing Salt Tolerance.

Plants (Basel, Switzerland)·2026
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Aug 25, 2025

Simulation, Fabrication and Characterization of THz Metamaterial Absorbers
13:44

Simulation, Fabrication and Characterization of THz Metamaterial Absorbers

Published on: December 27, 2012

15.4K

Multiresolution spectrally-encoded terahertz reflection imaging through a highly diffusive cloak.

Mahmoud E Khani, Zachery B Harris, Mengkun Liu

    Optics Express
    |October 15, 2022
    PubMed
    Summary
    This summary is machine-generated.

    This study introduces a new computational method using terahertz time-domain spectroscopy to map chemicals through scattering media. The technique reconstructs spectral images with high resolution, enabling material characterization.

    More Related Videos

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
    09:25

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

    Published on: August 22, 2018

    12.6K
    Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors
    08:56

    Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors

    Published on: April 5, 2020

    11.1K

    Related Experiment Videos

    Last Updated: Aug 25, 2025

    Simulation, Fabrication and Characterization of THz Metamaterial Absorbers
    13:44

    Simulation, Fabrication and Characterization of THz Metamaterial Absorbers

    Published on: December 27, 2012

    15.4K
    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy
    09:25

    Agarose-based Tissue Mimicking Optical Phantoms for Diffuse Reflectance Spectroscopy

    Published on: August 22, 2018

    12.6K
    Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors
    08:56

    Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors

    Published on: April 5, 2020

    11.1K

    Area of Science:

    • Optics and Photonics
    • Spectroscopy
    • Computational Imaging

    Background:

    • Turbid media with inhomogeneous particles cause significant imaging and spectroscopy challenges.
    • Scattering distorts wavefronts, leading to smeared images and obscuring chemical spectral fingerprints.
    • Accurate chemical imaging and material characterization are hindered by diffusive media.

    Purpose of the Study:

    • To develop a novel computational technique for spatially- and spectrally-resolved chemical mapping through diffusive media.
    • To overcome scattering-induced artifacts in imaging and spectroscopy.
    • To enable accurate material characterization in turbid environments.

    Main Methods:

    • Utilized terahertz time-domain spectroscopy (THz-TDS).
    • Applied maximal overlap discrete wavelet transform (MODWT) for multiresolution spectral decomposition of THz extinction coefficients.
    • Defined and calculated a "bimodality coefficient spectrum" using spectral image skewness and kurtosis.

    Main Results:

    • Demonstrated broadband wavelet-based reconstruction of the bimodality coefficient spectrum.
    • Successfully resolved signature resonant frequencies through scattering layers.
    • Achieved spectral images with diffraction-limited resolution.

    Conclusions:

    • The developed THz-TDS technique effectively creates chemical maps through diffusive media.
    • The bimodality coefficient spectrum analysis enables resolution of resonant frequencies despite scattering.
    • This approach offers potential for stand-off material characterization, nondestructive testing, and biological imaging.